壮观链霉菌中壮观菌素生物合成基因簇的克隆与异源表达
Cloning and heterologous expression of the spectinabilin biosynthetic gene cluster from Streptomyces spectabilis.
作者信息
Choi Yoo Seong, Johannes Tyler W, Simurdiak Michael, Shao Zengyi, Lu Haige, Zhao Huimin
机构信息
Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL 61801, USA.
出版信息
Mol Biosyst. 2010 Feb;6(2):336-8. doi: 10.1039/b923177c. Epub 2009 Dec 22.
Spectinabilin is a rare nitrophenyl-substituted polyketide metabolite. Here we report the cloning and heterologous expression of the spectinabilin gene cluster from Streptomyces spectabilis. Unexpectedly, this gene cluster is evolutionarily closer to the aureothin gene cluster than to the spectinabilin gene cluster from Streptomyces orinoci. Moreover, the two nearly identical spectinabilin gene clusters use a distinctly different regulation mechanism.
壮观菌素是一种罕见的硝基苯基取代聚酮类代谢产物。在此，我们报道了从壮观链霉菌中克隆并异源表达壮观菌素基因簇。出乎意料的是，该基因簇在进化上与金霉素基因簇的亲缘关系比与来自橄榄链霉菌的壮观菌素基因簇更近。此外，这两个几乎相同的壮观菌素基因簇采用截然不同的调控机制。
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