de Bruin Tanya, de Rooster Hilde, van Bree Henri, Duchateau Luc, Cox Eric
Department of Diagnostic Imaging of Domestic Animals, Faculty of Veterinary Medicine, University of Ghent, Salisburylaan 133, 9820 Merelbeke, Belgium.
Am J Vet Res. 2007 Sep;68(9):953-61. doi: 10.2460/ajvr.68.9.953.
To examine mRNA expression of cytokines in synovial fluid (SF) cells from dogs with cranial cruciate ligament (CrCL) rupture and medial patellar luxation (MPL) and determine mRNA expression for 3 joints (affected stifle, unaffected contralateral stifle, and left shoulder joints) in dogs with unilateral CrCL rupture.
29 stifle joints with CrCL rupture (29 dogs), 8 stifle joints with MPL (7 dogs), and 24 normal stifle joints (16 clinically normal dogs).
Immediately before reconstructive surgery, SF was aspirated from the cruciate-deficient stifle joint or stifle joint with MPL. Fourteen of 29 dogs had unilateral CrCL rupture; SF was also aspirated from the unaffected contralateral stifle joint and left shoulder joint. Those 14 dogs were examined 6 and 12 months after reconstructive surgery. Total RNA was extracted from SF cells and reverse transcription-PCR assay was performed to obtain cDNA. Canine-specific cytokine mRNA expression was determined by use of a real-time PCR assay.
Interleukin (IL)-8 and -10 and interferon-gamma expression differed significantly between dogs with arthropathies and dogs with normal stifle joints. For the 14 dogs with unilateral CrCL rupture, a significant difference was found for IL-8 expression. Before reconstructive surgery, IL-8 expression differed significantly between the affected stifle joint and left shoulder joint or contralateral stifle joint. Six months after surgery, IL-8 expression was significantly increased in the unaffected contralateral stifle joint, compared with the shoulder joint.
No conclusions can be made regarding the role of the examined cytokines in initiation of CrCL disease.
检测患有颅交叉韧带（CrCL）断裂和内侧髌骨脱位（MPL）的犬关节滑液（SF）细胞中细胞因子的mRNA表达，并确定单侧CrCL断裂犬的3个关节（患侧 stifle关节、对侧未受影响的stifle关节和左肩 关节）的mRNA表达。
29个患有CrCL断裂的stifle关节（29只犬）、8个患有MPL的stifle关节（7只犬）和24个正常的stifle关节（16只临床正常的犬）。
在重建手术前，立即从交叉韧带缺失的stifle关节或患有MPL的stifle关节抽取滑液。29只犬中有14只患有单侧CrCL断裂；还从未受影响的对侧stifle关节和左肩 关节抽取滑液。对这14只犬在重建手术后6个月和12个月进行检查。从SF细胞中提取总RNA，并进行逆转录聚合酶链反应（RT-PCR）检测以获得cDNA。使用实时PCR检测法测定犬特异性细胞因子mRNA表达。
患有关节病的犬与正常stifle关节的犬之间，白细胞介素（IL）-8、-10和干扰素-γ的表达存在显著差异。对于14只单侧CrCL断裂的犬，IL-8表达存在显著差异。在重建手术前，患侧stifle关节与左肩 关节或对侧stifle关节之间的IL-8表达存在显著差异。手术后6个月，与肩关节相比，未受影响的对侧stifle关节中IL-8表达显著增加。
关于所检测的细胞因子在CrCL疾病起始中的作用尚无定论。
