Venable Rachel O, Stoker Aaron M, Cook Cristi R, Cockrell Mary K, Cook James L
Comparative Orthopaedic Laboratory, Veterinary Medical Teaching Hospital, College of Veterinary Medicine University of Missouri, Columbia, MO 65211, USA.
Am J Vet Res. 2008 Dec;69(12):1569-73. doi: 10.2460/ajvr.69.12.1569.
To determine the quantity (concentration) and quality (molecular weight) of synovial fluid hyaluronan with respect to presence and severity of osteoarthritis in stifle joints of dogs.
21 purpose-bred dogs and 6 clinically affected large-breed dogs (cranial cruciate ligament [CrCL] disease with secondary osteoarthritis).
Research dogs underwent arthroscopic surgery in 1 stifle joint to induce osteoarthritis via CrCL transection (CrCLt; n=5 stifle joints), femoral condylar articular cartilage groove creation (GR; 6), or meniscal release (MR; 5); 5 had sham surgery (SH) performed. Contralateral stifle joints (n=21) were used as unoperated control joints. Synovial fluid was obtained from research dogs at time 0 and 12 weeks after surgery and from clinically affected dogs prior to surgery. All dogs were assessed for lameness, radiographic signs of osteoarthritis, and pathologic findings on arthroscopy as well as for quantity and quality of hyaluronan.
Clinically affected dogs had significantly greater degrees of pathologic findings, compared with dogs with surgically induced osteoarthritis (ie, those with CrCLt, GR, and MR stifle joints), and with respect to lameness scores, radiographic signs of osteoarthritis, pathologic findings on arthroscopy, and synovial fluid hyaluronan concentration. Synovial fluid from stifle joints of dogs with surgically induced osteoarthritis had hyaluronan bands at 35 kd on western blots that synovial fluid from SH and clinically affected stifle joints did not.
Synovial fluid hyaluronan quantity and quality were altered in stifle joints of dogs with osteoarthritis, compared with control stifle joints. A specific hyaluronan protein fragment may be associated with early pathologic changes in affected joints.
确定犬 stifle 关节骨关节炎的存在及严重程度与滑液透明质酸的量（浓度）和质（分子量）之间的关系。
21 只专门培育的犬和 6 只临床患病的大型犬（患有继发骨关节炎的前十字韧带[CrCL]疾病）。
研究用犬在 1 个 stifle 关节接受关节镜手术，通过切断 CrCL（CrCLt；n = 5 个 stifle 关节）、股骨髁关节软骨沟创建（GR；6 个）或半月板松解（MR；5 个）来诱导骨关节炎；5 只接受假手术（SH）。对侧 stifle 关节（n = 21 个）用作未手术的对照关节。在术后 0 周和 12 周从研究用犬获取滑液，并在手术前从临床患病犬获取滑液。对所有犬进行跛行评估、骨关节炎的放射学征象、关节镜检查的病理结果以及透明质酸的量和质的评估。
与手术诱导骨关节炎的犬（即患有 CrCLt、GR 和 MR stifle 关节的犬）相比，临床患病犬在病理结果、跛行评分、骨关节炎的放射学征象、关节镜检查的病理结果以及滑液透明质酸浓度方面的程度明显更高。手术诱导骨关节炎犬的 stifle 关节滑液在蛋白质印迹上有 35 kd 的透明质酸条带，而 SH 和临床患病 stifle 关节的滑液没有。
与对照 stifle 关节相比，骨关节炎犬的 stifle 关节滑液透明质酸的量和质发生了改变。一种特定的透明质酸蛋白片段可能与受影响关节的早期病理变化有关。
