固定在电极上的新生血红素蛋白的过氧化物酶活性。
Peroxidase activity of de novo heme proteins immobilized on electrodes.
作者信息
Das Aditi, Hecht Michael H
机构信息
Department of Chemistry, Princeton University, Princeton NJ 08544-1009, USA.
出版信息
J Inorg Biochem. 2007 Nov;101(11-12):1820-6. doi: 10.1016/j.jinorgbio.2007.07.024. Epub 2007 Jul 27.
Abstract
De novo proteins from designed combinatorial libraries were bound to heme terminated gold electrodes. The novel heme proteins were shown to possess peroxidase activity, and this activity was compared to that of horseradish peroxidase and bovine serum albumin when immobilized in a similar fashion. The various designed proteins from the libraries displayed distinctly different levels of peroxidase activity, thereby demonstrating that the sequence and structure of a designed protein can exert a substantial effect on the peroxidase activity of immobilized heme.
摘要
来自设计组合文库的从头合成蛋白质与血红素修饰的金电极结合。这些新型血红素蛋白显示出具有过氧化物酶活性，并且当以类似方式固定时，将这种活性与辣根过氧化物酶和牛血清白蛋白的活性进行了比较。文库中各种设计的蛋白质表现出明显不同水平的过氧化物酶活性，从而表明设计蛋白质的序列和结构可以对固定化血红素的过氧化物酶活性产生重大影响。
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