Fukuda Miho, Yamauchi Hiroshi, Yamamoto Hitoshi, Aminaka Masahito, Murakami Hiroshi, Kamiyama Noriko, Miyamoto Yusaku, Koitabashi Yasushi
Department of Pediatrics, St. Marianna University School of Medicine, Kawasaki, Japan.
Brain Dev. 2008 Feb;30(2):131-6. doi: 10.1016/j.braindev.2007.07.005. Epub 2007 Sep 4.
Urinary and cerebrospinal fluid (CSF) levels of 8-hydroxydeoxyguanosine (8-OHdG) were examined to estimate the relevance of oxidative stress in children with brain damage. Urinary 8-OHdG levels were measured in 51 children with various forms of central nervous system (CNS) disorders (status epilepticus [SE], hypoxic-ischemic encephalopathy [HIE], CNS infections and chronic epilepsy) and these levels were compared with those in 51 healthy children. CSF 8-OHdG levels were measured in 25 children with brain damage and in 19 control subjects. In addition, urinary and CSF levels of 8-OHdG were compared between the children with brain damage and healthy children. Finally, the relationship between urinary and CSF levels of 8-OHdG was determined in 12 children that provided both urinary and CSF samples. Our results showed that urinary 8-OHdG levels in children with HIE and CNS infections were higher than those of controls (Steel test; p < 0.05 and p < 0.05, respectively) and that CSF 8-OHdG levels were higher in children with SE, HIE, and CNS infections than in control subjects (Steel test; p < 0.01, 0.05 and 0.05, respectively). In addition, a positive correlation between the levels of urinary and CSF 8-OHdG was noted in the 12 children that provided both CSF and urinary samples (Spearman's rank correlation; rho = 0.82, p < 0.01). Further, we observed changes in the urinary 8-OHdG in a patient with HHV-6 encephalopathy, and found that the changes correlated well with the patient's clinical condition. These results suggest that oxidative stress is strongly related to acute brain damage in children, and that 8-OHdG is a useful marker of brain damage. Therefore, repeated measurements of urinary 8-OHdG may be helpful in estimating the extent of brain damage.
检测了尿和脑脊液（CSF）中8-羟基脱氧鸟苷（8-OHdG）的水平，以评估氧化应激与脑损伤儿童的相关性。测量了51例患有各种形式中枢神经系统（CNS）疾病（癫痫持续状态[SE]、缺氧缺血性脑病[HIE]、CNS感染和慢性癫痫）儿童的尿8-OHdG水平，并将这些水平与51例健康儿童的水平进行比较。测量了25例脑损伤儿童和19例对照受试者的脑脊液8-OHdG水平。此外，还比较了脑损伤儿童与健康儿童尿和脑脊液中8-OHdG的水平。最后，在12例同时提供尿样和脑脊液样本的儿童中确定了尿和脑脊液中8-OHdG水平之间的关系。我们的结果表明，患有HIE和CNS感染的儿童尿8-OHdG水平高于对照组（Steel检验；分别为p<0.05和p<0.05），患有SE、HIE和CNS感染的儿童脑脊液8-OHdG水平高于对照受试者（Steel检验；分别为p<0.01、0.05和0.05）。此外，在12例同时提供脑脊液和尿样的儿童中，尿和脑脊液8-OHdG水平之间存在正相关（Spearman等级相关性；rho=0.82，p<0.01）。此外，我们观察到1例HHV-6脑病患者尿8-OHdG的变化，并发现这些变化与患者的临床状况密切相关。这些结果表明，氧化应激与儿童急性脑损伤密切相关，8-OHdG是脑损伤的一个有用标志物。因此，重复测量尿8-OHdG可能有助于评估脑损伤的程度。
