Li Jian-Hua, Fu Ying, Chen Li-Yu, Dai Gan, Luo Min-Hua, Yang Tao
Department of Microbiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2007 Aug;32(4):551-6.
To determine whether U(251) cells are permissive for human cytomegalovirus (HCMV), and to investigate the characteristics of temporal expression of proteins IE1 and pp65.
U(251) cells were infected with HCMV, and then the cells were observed under the transmission electronic microscope, and the viral nucleic acid was detected by PCR, and the expression levels of IE1 and pp65 were analyzed by immunohistochemical assay with anti-IE1 monoclonal antibody and anti-pp65 monoclonal antibody at various time spost infection.
Morphological changes of the infected cells appeared under the transmission electron microscope. The viral nucleic acid was detected successfully by PCR. The expression of IE1 was detected firstly at 4h post infection, and reached a peak within 14h, and then decreased. The incoming pp65 was detected at 1h, the low expression levels of pp65 were detected firstly at 4h, and they could remain relatively constant through 96 h, but the maximum expression occurred at 120 h.
Human glioma U(251) cells are permissive for HCMV, the temporal cascade of HCMV gene expression can be observed in the infected U(251) cells, but it is delayed obviously in the human fibroblast.
确定U(251)细胞是否对人巨细胞病毒（HCMV）易感，并研究IE1和pp65蛋白的时间表达特征。
用HCMV感染U(251)细胞，然后在透射电子显微镜下观察细胞，并通过PCR检测病毒核酸，在感染后的不同时间用抗IE1单克隆抗体和抗pp65单克隆抗体通过免疫组织化学分析检测IE1和pp65的表达水平。
在透射电子显微镜下观察到感染细胞的形态学变化。通过PCR成功检测到病毒核酸。感染后4小时首次检测到IE1的表达，并在14小时内达到峰值，然后下降。在1小时时检测到传入的pp65，在4小时时首次检测到pp65的低表达水平，并且它们在96小时内可以保持相对恒定，但最大表达出现在120小时。
人胶质瘤U(251)细胞对HCMV易感，在感染的U(251)细胞中可以观察到HCMV基因表达的时间级联，但在人成纤维细胞中明显延迟。
