中枢神经系统肿瘤中 HCMV 成分的存在是否为胶质瘤特有的现象？

Is the presence of HCMV components in CNS tumors a glioma-specific phenomenon?

机构信息

Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.

Department of Infant Ward, Children's Hospital Affiliated of Zhengzhou University, Zhengzhou, 450052, China.

出版信息

Virol J. 2019 Aug 1;16(1):96. doi: 10.1186/s12985-019-1198-5.

DOI:10.1186/s12985-019-1198-5

PMID:31370833

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6670132/

Abstract

BACKGROUND

Human cytomegalovirus (HCMV) has been associated with malignant gliomas. The purpose of the present study was to investigate the presence of HCMV in common non-glial tumors of the central nervous system (CNS) and to determine whether it is a glioma-specific phenomenon.

METHODS

Using HCMV-specific immunohistochemical staining, HCMV proteins IE1-72 and pp65 were examined in 65 meningiomas (benign, atypical and malignant), 45 pituitary adenomas, 20 cavernous hemangiomas, and 30 metastatic carcinomas specimens. HCMV DNA was also measured in these tumor tissues and the peripheral blood from patients using nested PCR.

RESULTS

In meningioma, IE1-72 was detected in 3.1% (2/65) and pp65 was detected in 4.6% (3/65), whereas no IE1-72 and pp65 were detected in atypical and malignant meningioma. A low level of IE1-72 immunoreactivity 6.7% (2/30) was detected in metastatic carcinoma; pp65 was not detected. No HCMV components were detected in pituitary adenoma and cavernous hemangioma. The results of immunohistochemical staining were confirmed by HCMV-specific PCR. HCMV DNA was not detected in the peripheral blood of the non-glial CNS tumors patients.

CONCLUSIONS

Our results demonstrate that the presence of HCMV components is not an entirely glioma-specific phenomenon, and that HCMV is present in a low percentage in some non-glioma CNS tumors. Comparing HCMV-positive non-glial CNS tumors with HCMV-positive gliomas may cast light on the mechanism and role of HCMV in CNS tumors.

摘要

背景

人巨细胞病毒（HCMV）与恶性神经胶质瘤有关。本研究旨在探讨 HCMV 在中枢神经系统（CNS）常见非神经胶质瘤肿瘤中的存在情况，并确定其是否为神经胶质瘤特异性现象。

方法

使用 HCMV 特异性免疫组织化学染色，检测 65 例脑膜瘤（良性、非典型和恶性）、45 例垂体腺瘤、20 例海绵状血管瘤和 30 例转移性癌标本中的 HCMV 蛋白 IE1-72 和 pp65。使用巢式 PCR 测量这些肿瘤组织和患者外周血中的 HCMV DNA。

结果

在脑膜瘤中，IE1-72 的检出率为 3.1%（2/65），pp65 的检出率为 4.6%（3/65），而在非典型和恶性脑膜瘤中未检出 IE1-72 和 pp65。转移性癌中低水平的 IE1-72 免疫反应性为 6.7%（2/30）；未检出 pp65。垂体腺瘤和海绵状血管瘤均未检出 HCMV 成分。免疫组织化学染色结果通过 HCMV 特异性 PCR 得到证实。非神经胶质瘤 CNS 肿瘤患者的外周血中未检测到 HCMV DNA。