Xie Min, Yang Yu-Jia, Liu Chen-Tao, Wang Qing-Hong, Wang Xia, Yu Xiao-He
Department of Paediatrics, Xiangya Hospital,Central South University, Changsha 410008, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2007 Aug;32(4):557-62.
To investigate the effect of brain tissue extracts in neonate rats with hypoxic-ischemic brain damage (HIBD) on the differentiation of bone marrow stromal cells (BMSCs) into neural cells.
Fifteen 7-day-old neonate rats were induced HIBD by left carotid artery ligation and hypoxia exposure, and another 15-day-old neonate rats were served as normal rats. The left and right brain tissue extracts of the normal and HIBD rats were prepared 24 h after the HIBD (8-day old), 72 h after the HIBD (10-day old), and 7 d after the HIBD (14-day old), respectively (n=5). The rat BMSCs of passage 3-5 were cultured in the medium with or without previous brain tissue extracts. The expressions of neuron specific enolase (NSE), glial fibrillary acidic protein (GFAP) and O(4) marked oligodendrocyte were detected after 3 days by immunocytochemistry.
The expressions of NSE, GFAP and O(4) of BMSCs cultured in the medium with left or right brain tissue extracts of different day old normal rats were higher than those of BMSCs cultured without the extracts, respectively (P<0.01), and the expressions of NSE, GFAP and O(4) of BMSCs cultured in the medium with left brain tissue extracts of 8 day old and 10 day old HIBD rats were higher than those of BMSCs cultured with right brain tissue extracts of the same day HIBD rats and BMSCs cultured with left or right brain tissue extracts of the same day normal rats (P<0.01 or P<0.05). The expressions of NSE, GFAP and O(4) of BMSCs cultured in the medium with left brain tissue extracts of 8-day-old HIBD rats were higher than those of BMSCs cultured with left brain tissue extracts of 10-day-old and 14-day-old HIBD rats (P<0.01 or P<0.05).
The brain tissue extracts of normal and HIBD rats can induce BMSCS into neural cells, and the damaged brain tissue extracts of 8-day-old HIBD rats is the best inductor.
探讨脑组织提取物对缺氧缺血性脑损伤（HIBD）新生大鼠骨髓基质细胞（BMSCs）向神经细胞分化的影响。
15只7日龄新生大鼠通过左侧颈动脉结扎和缺氧诱导HIBD，另15只7日龄新生大鼠作为正常大鼠。分别在HIBD后24小时（8日龄）、72小时（10日龄）和7天（14日龄）制备正常大鼠和HIBD大鼠的左右脑组织提取物（n = 5）。将第3 - 5代大鼠BMSCs培养于含或不含先前脑组织提取物的培养基中。3天后通过免疫细胞化学检测神经元特异性烯醇化酶（NSE）、胶质纤维酸性蛋白（GFAP）和O(4)标记的少突胶质细胞的表达。
不同日龄正常大鼠左右脑组织提取物培养基中培养的BMSCs的NSE、GFAP和O(4)表达分别高于无提取物培养基中培养的BMSCs（P < 0.01），8日龄和10日龄HIBD大鼠左侧脑组织提取物培养基中培养的BMSCs的NSE、GFAP和O(4)表达高于同日龄HIBD大鼠右侧脑组织提取物培养基中培养的BMSCs以及同日龄正常大鼠左右脑组织提取物培养基中培养的BMSCs（P < 0.01或P < 0.05）。8日龄HIBD大鼠左侧脑组织提取物培养基中培养的BMSCs的NSE、GFAP和O(4)表达高于10日龄和14日龄HIBD大鼠左侧脑组织提取物培养基中培养的BMSCs（P < 0.01或P < 0.05）。
正常大鼠和HIBD大鼠的脑组织提取物均可诱导BMSCs向神经细胞分化，8日龄HIBD大鼠受损脑组织提取物诱导效果最佳。
