脑室注射人牙髓干细胞可改善新生大鼠缺氧缺血性脑损伤。
Intraventricular injection of human dental pulp stem cells improves hypoxic-ischemic brain damage in neonatal rats.
机构信息
Department of Pediatrics, Xiangya Hospital, Central South University, Changsha, China.
出版信息
PLoS One. 2013 Jun 14;8(6):e66748. doi: 10.1371/journal.pone.0066748. Print 2013.
OBJECTIVE
To investigate the effect of intraventricular injection of human dental pulp stem cells (DPSCs) on hypoxic-ischemic brain damage (HIBD) in neonatal rats.
METHODS
Thirty-six neonatal rats (postnatal day 7) were assigned to control, HIBD, or HIBD+DPSC groups (n = 12 each group). For induction of HIBD, rats underwent left carotid artery ligation and were exposed to 8% to 10% oxygen for 2 h. Hoechst 33324-labeled human DPSCs were injected into the left lateral ventricle 3 days after HIBD. Behavioral assays were performed to assess hypoxic-ischemic encephalopathy (HIE), and on postnatal day 45, DPSC survival was assessed and expression of neural and glial markers was evaluated by immunohistochemistry and Western blot.
RESULTS
The HIBD group showed significant deficiencies compared to control on T-maze, radial water maze, and postural reflex tests, and the HIBD+DPSC group showed significant improvement on all behavioral tests. On postnatal day 45, Hoechst 33324-labeled DPSC nuclei were visible in the injected region and left cortex. Subsets of DPSCs showed immunostaining for neuronal (neuron-specific enolase [NSE], Nestin) and glial markers (glial fibrillary acidic protein [GFAP], O4). Significantly decreased staining/expression for NSE, GFAP, and O4 was found in the HBID group compared to control, and this was significantly increased in the HBID+DPSC group.
CONCLUSION
Intraventricular injection of human DPSCs improves HIBD in neonatal rats.
目的
研究脑室内注射人牙髓干细胞（DPSCs）对新生大鼠缺氧缺血性脑损伤（HIBD）的影响。
方法
将 36 只新生大鼠（生后 7 天）分为对照组、HIBD 组和 HIBD+DPSC 组（每组 12 只）。HIBD 模型通过结扎左侧颈总动脉并暴露于 8%至 10%的氧气中 2 小时来诱导。HIBD 后 3 天，将 Hoechst 33324 标记的人 DPSCs 注射到左侧侧脑室。通过 T 迷宫、放射状水迷宫和姿势反射试验评估缺氧缺血性脑病（HIE），并在生后第 45 天评估 DPSC 存活情况，通过免疫组织化学和 Western blot 评估神经和神经胶质标志物的表达。
结果
与对照组相比，HIBD 组在 T 迷宫、放射状水迷宫和姿势反射试验中表现出明显的缺陷，而 HIBD+DPSC 组在所有行为测试中均有明显改善。在生后第 45 天，可在注射区域和左侧皮质中观察到 Hoechst 33324 标记的 DPSC 核。DPSC 的亚群对神经元（神经元特异性烯醇化酶[NSE]，巢蛋白[Nestin]）和神经胶质标志物（神经胶质纤维酸性蛋白[GFAP]，O4）有免疫染色。与对照组相比，HIBD 组的 NSE、GFAP 和 O4 染色/表达明显减少，而 HIBD+DPSC 组则明显增加。
结论
脑室内注射人 DPSCs 可改善新生大鼠的 HIBD。
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