在一项针对激素替代疗法使用者的非雷特司汀与安慰剂随机化学预防试验中，对乳腺细针穿刺进行计算机辅助图像分析。
Computer-assisted image analysis of breast fine needle aspiration in a randomized chemoprevention trial of fenretinide vs. placebo in HRT users.
作者信息
Serrano Davide, Gandini Sara, Mariani Luigi, Bonanni Bernardo, Santinelli Alfredo, Guerrieri-Gonzaga Aliana, Pelosi Giuseppe, Cassano Enrico, Montironi Rodolfo, Decensi Andrea
机构信息
Division of Chemoprevention, European Institute of Oncology, Milan, Italy.
出版信息
Breast. 2008 Feb;17(1):91-7. doi: 10.1016/j.breast.2007.07.038. Epub 2007 Sep 4.
BACKGROUND
Digital nuclear morphometric analysis can capture subtle differences along neoplastic progression. Studies showed different profiles from normal to cancer lesions. Our goal is to utilize this method as biomarker in chemoprevention trials.
METHODS
Postmenopausal women were randomized to oral (CEE) or transdermal (E2) estrogen replacement therapy (ERT) in association with fenretinide or placebo. Ultrasound-guided fine needle aspiration (FNA) was performed at baseline and after 12 months in a subset of subjects.
RESULTS
Ten samples were analyzed by karyometry. E2 compared with CEE increased nuclear area (p=0.01). A similar pattern was observed for other DNA content and chromatin texture features. Fenretinide vs. placebo, increased nuclear area and shape while decreased slope, peak and entropy.
CONCLUSION
Preliminary results indicate that nuclear morphometry is feasible on FNA samples. ERT and fenretinide induced significant karyometric changes. These results support further investigation of this procedure as surrogate biomarker in chemoprevention trial.
背景
数字核形态计量分析能够捕捉肿瘤进展过程中的细微差异。研究显示了从正常病变到癌症病变的不同特征。我们的目标是在化学预防试验中将该方法用作生物标志物。
方法
绝经后女性被随机分为接受口服（结合雌激素）或经皮（雌二醇）雌激素替代疗法（ERT），并联合维甲酸或安慰剂。在基线时以及12个月后，对一部分受试者进行超声引导下细针穿刺抽吸（FNA）。
结果
通过核测量法分析了10个样本。与结合雌激素相比，雌二醇增加了核面积（p = 0.01）。在其他DNA含量和染色质纹理特征方面也观察到了类似的模式。维甲酸与安慰剂相比，增加了核面积和形状，同时降低了斜率、峰值和熵。
结论
初步结果表明，核形态计量学在FNA样本上是可行的。ERT和维甲酸诱导了显著的核测量变化。这些结果支持在化学预防试验中进一步研究将该程序作为替代生物标志物。
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