贪铜菌H16中的DUF81蛋白TauE，是C2磺酸盐代谢中的一种亚硫酸盐转运蛋白。
The DUF81 protein TauE in Cupriavidus necator H16, a sulfite exporter in the metabolism of C2 sulfonates.
作者信息
Weinitschke Sonja, Denger Karin, Cook Alasdair M, Smits Theo H M
机构信息
Department of Biology, The University, D-78457 Konstanz, Germany.
出版信息
Microbiology (Reading). 2007 Sep;153(Pt 9):3055-3060. doi: 10.1099/mic.0.2007/009845-0.
The degradation of taurine, isethionate and sulfoacetate in Cupriavidus necator (Ralstonia eutropha) H16 was shown by enzyme assays to be inducible, and each pathway involved sulfoacetaldehyde, which was subject to phosphatolysis by a common sulfoacetaldehyde acetyltransferase (Xsc, H16_B1870) to yield acetyl phosphate and sulfite. The neighbouring genes encoded phosphate acetyltransferase (Pta, H16_B1871) and a hypothetical protein [domain of unknown function (DUF)81, H16_B1872], with eight derived transmembrane helices. RT-PCR showed inducible transcription of these three genes, and led to the hypothesis that H16_B1872 and orthologous proteins represent a sulfite exporter, which was named TauE.
通过酶分析表明，贪铜菌（嗜麦芽窄食单胞菌）H16中牛磺酸、羟乙磺酸盐和磺基乙酸盐的降解是可诱导的，并且每条途径都涉及磺基乙醛，磺基乙醛会被一种常见的磺基乙醛乙酰转移酶（Xsc，H16_B1870）进行磷酸解作用，生成乙酰磷酸和亚硫酸盐。相邻基因编码磷酸乙酰转移酶（Pta，H16_B1871）和一种假定蛋白[功能未知结构域（DUF）81，H16_B1872]，该假定蛋白有八个推导的跨膜螺旋。逆转录聚合酶链反应显示这三个基因的转录是可诱导的，并由此推测H16_B1872及其直系同源蛋白代表一种亚硫酸盐转运蛋白，将其命名为TauE。
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