Huang Yan, Zhou Zhenwen, Hu Xuchu, Wei Quande, Xu Jin, Wu Zhongdao, Yu Xinbing
Department of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, 74th, Zhongshan Road 2, Guangzhou, 510089, People's Republic of China.
Parasitol Res. 2007 Dec;102(1):77-81. doi: 10.1007/s00436-007-0728-z. Epub 2007 Sep 3.
The complementary DNA (cDNA) plasmid libraries of adult worm, metacercariae and egg of Clonorchis sinensis (C. sinensis) were constructed for researches on genomics and proteomics of C. sinensis. The full-length cDNA sequence encoding tegumental protein 31.8 kDa (CsTP31.8) was identified from the adult cDNA library. The cDNA sequence has been submitted to the GeneBank Database with accession number ABK60086. This novel cDNA sequence contains 828 bp with a putative open reading frame of 275 amino acids. The deduced amino acid sequence shows identity to membrane-associate antigens or tegumental antigens of other species. There were conserved calcium-binding EF hand and dynein light chain type 1 in the sequence. CsTP31.8 transcripts were detected in cDNA libraries of adult worm and metacercariae but not in that of egg. Recombinant CsTP31.8 was expressed and purified from Escherichia coli BL21 (DE3). CsTP31.8 was immunolocalized at the tegument of adult C. sinensis by using antirecombinant CsTP31.8 sera.
构建华支睾吸虫成虫、囊蚴和虫卵的互补DNA（cDNA）质粒文库，用于华支睾吸虫基因组学和蛋白质组学研究。从成虫cDNA文库中鉴定出编码31.8 kDa体表蛋白（CsTP31.8）的全长cDNA序列。该cDNA序列已提交至基因库数据库，登录号为ABK60086。这个新的cDNA序列包含828 bp，推定的开放阅读框为275个氨基酸。推导的氨基酸序列与其他物种的膜相关抗原或体表抗原具有同一性。序列中存在保守的钙结合EF手型和动力蛋白轻链1型。在成虫和囊蚴的cDNA文库中检测到CsTP31.8转录本，但在虫卵的cDNA文库中未检测到。重组CsTP31.8在大肠杆菌BL21（DE3）中表达并纯化。使用抗重组CsTP31.8血清将CsTP31.8免疫定位在华支睾吸虫成虫的体表。
