Singh S, Keller D J
Department of Chemistry, University of New Mexico, Albuquerque 87131.
Biophys J. 1991 Dec;60(6):1401-10. doi: 10.1016/S0006-3495(91)82177-4.
Membrane bilayers of dipalmitoyl phosphatidylcholine (DPPC) and dipalmitoyl phosphatidylethanolamine (DPPE) adsorbed to a freshly cleaved mica substrate have been imaged by Atomic Force Microscopy (AFM). The membranes were mounted for imaging by two methods: (a) by dialysis of a detergent solution of the lipid in the presence of the substrate material, and (b) by adsorption of lipid vesicles onto the substrate surface from a vesicle suspension. The images were taken in air, and show lipid bilayers adhering to the surface either in isolated patches or in continuous sheets, depending on the deposition conditions. Epifluorescence light-microscopy shows that the lipid is distributed on the substrate surfaces as seen in the AFM images. In some instances, when DPPE was used, whole, unfused vesicles, which were bound to the substrate, could be imaged by the AFM. Such membranes should be capable of acting as natural anchors for imaging membrane proteins by AFM.
已通过原子力显微镜（AFM）对吸附在刚劈开的云母基底上的二棕榈酰磷脂酰胆碱（DPPC）和二棕榈酰磷脂酰乙醇胺（DPPE）的膜双层进行了成像。通过两种方法将膜安装用于成像：（a）在存在基底材料的情况下对脂质的去污剂溶液进行透析，以及（b）从囊泡悬浮液中将脂质囊泡吸附到基底表面。图像是在空气中拍摄的，根据沉积条件，显示脂质双层以孤立斑块或连续片层的形式附着在表面。落射荧光显微镜显示，如AFM图像中所见，脂质分布在基底表面。在某些情况下，当使用DPPE时，结合到基底上的完整、未融合的囊泡可以通过AFM成像。这样的膜应该能够作为通过AFM对膜蛋白进行成像的天然锚定物。
