Murphy R M, Yarmush M L, Colton C K
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge.
Biopolymers. 1991 Oct;31(11):1289-95. doi: 10.1002/bip.360311107.
Physiological properties of soluble antigen-antibody (Ag-Ab) complexes depend in part on the size of the complexes. In previous work, the size distribution and structure of model Ag-Ab complexes were determined by electron microscopy. In this study, we used constrained regularization analysis of quasi-elastic light scattering data to estimate molecular weight distributions of model Ag-Ab complexes. A conformational model was necessary to determine appropriate correlations between molecular weight and diffusion coefficient, and to estimate particle structure factors. Porod-Kratky theory proved to be an adequate conformational model for these purposes. The molecular weight distributions determined by constrained regularization compared favorably with distributions obtained either by electron microscopy or by thermodynamic modeling.
可溶性抗原-抗体（Ag-Ab）复合物的生理特性部分取决于复合物的大小。在之前的工作中，通过电子显微镜确定了模型Ag-Ab复合物的大小分布和结构。在本研究中，我们使用准弹性光散射数据的约束正则化分析来估计模型Ag-Ab复合物的分子量分布。为了确定分子量与扩散系数之间的适当相关性，并估计颗粒结构因子，需要一个构象模型。事实证明，Porod-Kratky理论是用于这些目的的合适构象模型。通过约束正则化确定的分子量分布与通过电子显微镜或热力学建模获得的分布相比具有优势。
