Gorskaia Iu F, Fridenshteĭn A Ia, Golovanova T A
Biull Eksp Biol Med. 1991 Dec;112(12):615-7.
Purified mouse IL-1 at doses 15-100 mu/ml inhibits the growth of stromal clonogenic cells /CFU-f/ both in full bone marrow cell cultures /F-cultures/ and in adherent bone marrow cell cultures /A-cultures/. Rec. human TNF-alpha inhibits growth of these cells at doses greater than 50 u/ml, but stimulates it /in 1.5 fold increase/at low doses /0.1-20 u/ml/ in cultures of both types. Rec. mouse IL-3 at doses 0.8-50 mu/ml slightly increases/in 1.6 fold increase/the in vitro growth of CFU-f and inhibits it at low doses in F-cultures. In A-cultures this factor stimulates CFU-f growth at all doses tested, but this stimulating effect takes place if only explantation density of mouse bone marrow cells in sufficiently high.
纯化的小鼠白细胞介素-1（IL-1）在15 - 100微克/毫升的剂量下，可抑制全骨髓细胞培养物（F-培养物）和贴壁骨髓细胞培养物（A-培养物）中基质克隆形成细胞/CFU-f/的生长。重组人肿瘤坏死因子-α（TNF-α）在剂量大于50单位/毫升时抑制这些细胞的生长，但在两种类型的培养物中，低剂量（0.1 - 20单位/毫升）时可刺激其生长（增加1.5倍）。重组小鼠白细胞介素-3（IL-3）在0.8 - 50微克/毫升的剂量下，可轻微增加（增加1.6倍）CFU-f的体外生长，并在F-培养物中低剂量时抑制其生长。在A-培养物中，该因子在所有测试剂量下均刺激CFU-f生长，但只有当小鼠骨髓细胞的接种密度足够高时，才会产生这种刺激作用。
