Latsinik N V, Fridenshteĭn A Ia, Gorskaia Iu F, Moskvina I L, Iuĭ Kh Ch
Biull Eksp Biol Med. 1990 Nov;110(11):519-22.
CFU-f-derived stromal colony formation was accomplished in adherent marrow cell cultures (AMCC) with serum-rich medium. It turned out to require additional stimulation by hemopoietic feeder cells: by irradiated marrow cells and spleen cells if they possess megakaryocytes and platelets or by platelets from the blood. PDGF, EGF and IL-3 did not substitute the colony stimulating activity of feeder cells. Thymus, lymph node cells and blood leucocytes had no colony stimulating activity. At low oxygen concentrations which improve colony formation the stimulating activity of hemopoietic feeder cells was expressed, as well. Thus, CFU-f colony formation depends on stimulation by hemopoietic cells in addition to serum growth factors. In full populations of marrow cells the CFU-f colony formation is stimulated by marrow cells which accompany the CFU-f.
在富含血清的培养基中，通过贴壁骨髓细胞培养（AMCC）实现了CFU-f衍生的基质集落形成。结果表明，它需要造血饲养细胞的额外刺激：如果它们拥有巨核细胞和血小板，则通过照射过的骨髓细胞和脾细胞，或者通过血液中的血小板。血小板衍生生长因子（PDGF）、表皮生长因子（EGF）和白细胞介素-3（IL-3）不能替代饲养细胞的集落刺激活性。胸腺、淋巴结细胞和血液白细胞没有集落刺激活性。在改善集落形成的低氧浓度下，造血饲养细胞的刺激活性也会表现出来。因此，CFU-f集落形成除了依赖血清生长因子外，还依赖造血细胞的刺激。在完整的骨髓细胞群体中，CFU-f集落形成受到伴随CFU-f的骨髓细胞的刺激。
