Dolce V, Fiermonte G, Messina A, Palmieri F
Department of Pharmaco-Biology, University of Bari, Italy.
DNA Seq. 1991;2(2):133-5. doi: 10.3109/10425179109039683.
We have isolated and characterized a full length cDNA clone encoding the precursor of the human heart mitochondrial phosphate carrier protein. The entire clone is 1330 bp in length with 5'- and 3'-untranslated regions of 48 and 184 bp, respectively. The open reading frame encodes the mature protein consisting of 312 amino acids, preceded by a presequence of 49 amino acids. The amino acid sequence of the mature human phosphate carrier is 93.6, 94.2 and 33.6% identical to that of the phosphate carrier from beef, rat and yeast, respectively. Like other mitochondrial transport proteins, the human phosphate carrier has a tripartite structure. Each of the three repeats contains two hydrophobic regions which presumably span the membrane in the form of alpha-helices.
我们已经分离并鉴定了一个编码人心肌线粒体磷酸盐载体蛋白前体的全长cDNA克隆。整个克隆长度为1330 bp，5'非翻译区和3'非翻译区分别为48 bp和184 bp。开放阅读框编码由312个氨基酸组成的成熟蛋白，其前面有一个49个氨基酸的前序列。成熟的人磷酸盐载体的氨基酸序列与牛肉、大鼠和酵母的磷酸盐载体的氨基酸序列分别有93.6%、94.2%和33.6%的同源性。与人其他线粒体转运蛋白一样，人磷酸盐载体具有三重结构。三个重复序列中的每一个都包含两个疏水区域，推测它们以α螺旋的形式跨越膜。
