编码线粒体三羧酸载体蛋白的牛cDNA的克隆与测序

Cloning and sequencing of the bovine cDNA encoding the mitochondrial tricarboxylate carrier protein.

作者信息

Iacobazzi V, De Palma A, Palmieri F

机构信息

Department of Pharmaco-Biology, University of Bari, Italy.

出版信息

Biochim Biophys Acta. 1996 Oct 2;1284(1):9-12. doi: 10.1016/0005-2736(96)00115-0.

DOI:10.1016/0005-2736(96)00115-0

PMID:8865808

Abstract

The tricarboxylate or citrate transporter protein (CTP) catalyzes the transport of citrate across the inner mitochondrial membrane by an exchange for malate or some other anionic metabolite. Using primers based on the rat liver cDNA sequence, overlapping cDNA clones encoding the bovine CTP were isolated from bovine liver poly(A+) cDNA. The entire bovine cDNA is 1151 bp in length with 5' and 3' untranslated regions of 7 and 204 bp, respectively. The open reading frame encodes the mature protein consisting of 298 amino acids, preceded by a presequence of 13 amino acids. The amino acid sequence of the mature bovine CTP is 95.6, 94.9, 32.2% identical to that of the citrate carrier from man, rat and yeast, respectively.

摘要

三羧酸或柠檬酸转运蛋白（CTP）通过与苹果酸或其他一些阴离子代谢物进行交换，催化柠檬酸穿过线粒体内膜的转运。使用基于大鼠肝脏cDNA序列的引物，从牛肝脏poly(A+) cDNA中分离出编码牛CTP的重叠cDNA克隆。完整的牛cDNA长度为1151 bp，5'和3'非翻译区分别为7 bp和204 bp。开放阅读框编码由298个氨基酸组成的成熟蛋白，前面有一个13个氨基酸的前序列。成熟牛CTP的氨基酸序列与人、大鼠和酵母的柠檬酸载体的氨基酸序列分别有95.6%、94.9%和32.2%的同源性。