红细胞乙酰胆碱酯酶的免疫测定

Immunological assay of erythrocyte acetylcholinesterase.

作者信息

Jones J W, Whittaker M, Braven J

机构信息

Blood Transfusion Department, Torbay Hospital, Plymouth, UK.

出版信息

Clin Chim Acta. 1991 Aug 30;200(2-3):175-81. doi: 10.1016/0009-8981(91)90088-t.

DOI:10.1016/0009-8981(91)90088-t

PMID:1777967

Abstract

An immunoassay for the quantitation of erythrocyte surface acetylcholinesterase is described; using a red cell suspension, bound mouse monoclonal acetylcholinesterase antibody is detected by an alkaline phosphatase conjugated rabbit anti mouse IgG. Extraction is not required. In addition, the activity of erythrocyte surface acetylcholinesterase using dithiobisnitrobenzoate to detect released thiocholine has been measured. The coefficient of variation for each method is 7%. Reference ranges have been established for healthy adults and cord blood.

摘要

本文描述了一种用于定量红细胞表面乙酰胆碱酯酶的免疫测定法；使用红细胞悬液，通过碱性磷酸酶偶联的兔抗小鼠IgG检测结合的小鼠单克隆乙酰胆碱酯酶抗体。无需提取。此外，还测定了使用二硫代双硝基苯甲酸检测释放的硫代胆碱时红细胞表面乙酰胆碱酯酶的活性。每种方法的变异系数为7%。已确定健康成年人和脐带血的参考范围。