Li J L, Li Y J, Chao Y J, Lin L X, Ouyang M H, Peng Y B, Chang W S
Department of Malaria Immunology, First Medical University of PLA, Guangzhou, The People's Republic of China.
Cytobios. 1991;68(272):15-22.
Cholesterol, a major lipid component of the plasma membrane, is thought to have profound effects on the structure and function of cells. Most animal tissues are capable of synthesizing cholesterol de novo from acetate; however, there are relatively few mammalian cells in vitro expressing an absolute requirement for an exogenous source of cholesterol. In this paper, it was shown that both IR983F (983) rat myeloma cells and P3X63-Ag8-U1 (P3U1) mouse myeloma cells which had been cultivated in serum-free medium containing cholesterol for more than 6 months still required cholesterol in vitro for growth in serum-free medium. Optimal growth of 983 and P3U1 occurred in cholesterol concentrations of 15 and 5 micrograms/ml, respectively. Moreover, it was demonstrated that the cholesterol could be replaced by human low density lipoprotein in a concentration of 10 micrograms/ml but not by mevalonic acid lactone. In contrast to the parental myeloma cells, hybridoma cells derived from the mouse myeloma cells which had been cultivated in serum-free medium containing cholesterol for more than 6 months did not require cholesterol.
胆固醇是质膜的主要脂质成分，被认为对细胞的结构和功能有深远影响。大多数动物组织能够从乙酸盐从头合成胆固醇；然而，在体外相对较少的哺乳动物细胞表现出对胆固醇外源供应的绝对需求。本文表明，在含胆固醇的无血清培养基中培养超过6个月的IR983F（983）大鼠骨髓瘤细胞和P3X63 - Ag8 - U1（P3U1）小鼠骨髓瘤细胞，在体外无血清培养基中生长仍需要胆固醇。983和P3U1的最佳生长分别发生在胆固醇浓度为15微克/毫升和5微克/毫升时。此外，已证明胆固醇可以被浓度为10微克/毫升的人低密度脂蛋白替代，但不能被甲羟戊酸内酯替代。与亲本骨髓瘤细胞不同，从在含胆固醇的无血清培养基中培养超过6个月的小鼠骨髓瘤细胞衍生的杂交瘤细胞不需要胆固醇。
