Bricker L A, Kozlovskis P L, Levey G S
Metabolism. 1976 Apr;25(4):477-81. doi: 10.1016/0026-0495(76)90081-0.
Many investigators have reported that adenosine 3',5'-monophosphate (cyclic AMP) at supraphysiologic concentrations (10(-4) M) suppresses lipogenesis in rat livers in vitro. The data in this report demonstrate that the phosphodiesterase inhibitor theophylline does not suppress sterol synthesis by itself nor does it potentiate the cyclic AMP-mediated suppression. In addition the cyclic AMP-mediated effect was not specific as many structurally-related compounds including adenosine triphosphate, adenosine 5'-diphosphate, adenosine 5'-monophosphate, adenosine, coenzyme A, HMG coenzyme A, and guanosine 3',5'-monophosphate suppressed sterol synthesis. The phenomenon of cyclic AMP-suppression of sterol synthesis does not fulfill two of the four criteria defined by Sutherland to assess whether a given metabolic effect is mediated by cyclic AMP. Therefore, we conclude that the cyclic AMP mediated suppression of sterologenesis is not a physiologic effect of the nucleotide.
许多研究人员报告称，超生理浓度（10⁻⁴ M）的3',5'-单磷酸腺苷（环磷酸腺苷）在体外可抑制大鼠肝脏中的脂肪生成。本报告中的数据表明，磷酸二酯酶抑制剂茶碱本身不会抑制固醇合成，也不会增强环磷酸腺苷介导的抑制作用。此外，环磷酸腺苷介导的效应并不具有特异性，因为许多结构相关的化合物，包括三磷酸腺苷、5'-二磷酸腺苷、5'-单磷酸腺苷、腺苷、辅酶A、HMG辅酶A和3',5'-单磷酸鸟苷，都能抑制固醇合成。环磷酸腺苷对固醇合成的抑制现象并不符合萨瑟兰定义的用于评估特定代谢效应是否由环磷酸腺苷介导的四个标准中的两个。因此，我们得出结论，环磷酸腺苷介导的对固醇生成的抑制不是该核苷酸的生理效应。
