采用酶法合成3-呋喃甲基青霉素。

Synthesis of 3-furylmethylpenicillin using an enzymatic procedure.

作者信息

Ferrero O, Reglero A, Martín-Villacorta J, Martínez-Blanco H, Luengo J M

机构信息

Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de León, Spain.

出版信息

FEMS Microbiol Lett. 1991 Sep 15;67(1):1-5. doi: 10.1016/0378-1097(91)90433-b.

DOI:10.1016/0378-1097(91)90433-b

PMID:1778415

Abstract

3-Furylmethylpenicillin was synthesized in vitro from 3-furylacetic acid, 6-aminopenicillanic acid (6-APA), CoA, ATP and Mg2+. The reaction was catalyzed in two steps by the enzymes phenyl-acetyl-CoA ligase (PCL) from Pseudomonas putida and acyl-CoA: 6-APA acyltransferase (AT) from Penicillium chrysogenum. PCL catalyzes the activation of 3-furylacetic acid to 3-furylacetyl-CoA (3-F-CoA) and AT acylates the amino group of 6-APA with the 3-furylacetyl moiety of 3-F-CoA, releasing CoA and 3-furylmethylpenicillin.

摘要

3-呋喃甲基青霉素是在体外由3-呋喃乙酸、6-氨基青霉烷酸（6-APA）、辅酶A、三磷酸腺苷（ATP）和镁离子合成的。该反应由恶臭假单胞菌的苯乙酰辅酶A连接酶（PCL）和产黄青霉的酰基辅酶A：6-APA酰基转移酶（AT）分两步催化。PCL催化3-呋喃乙酸活化为3-呋喃乙酰辅酶A（3-F-CoA），AT则用3-F-CoA的3-呋喃乙酰部分使6-APA的氨基酰化，释放出辅酶A和3-呋喃甲基青霉素。