Masuko M, Hosoi S, Hayakawa T
Tsukuba Research Laboratory, Hamamatsu Photonics K.K., Japan.
FEMS Microbiol Lett. 1991 Oct 1;67(2):231-8. doi: 10.1016/0378-1097(91)90359-i.
Using Escherichia coli as a model bacterium, we tested a photon-counting method for enumeration of bacteria. This method is based on the principle that microscopic sized luminous particles in a wide field can be directly detected and counted using a photon-counting TV camera without the use of a microscope. E. coli cells were labeled with peroxidase and luminescence induced by adding a luminol-based reaction mixture. The number of luminous spots in the TV images was in good agreement with the number of bacterial colonies grown from labeled cells. The results show that our method provides a rapid and easy microbial counting system for such purposes as clinical diagnosis, microbial analysis in food, and environmental assessment.
以大肠杆菌作为模式细菌，我们测试了一种用于细菌计数的光子计数方法。该方法基于这样的原理：无需使用显微镜，利用光子计数电视摄像机就可以直接检测和计数宽视野中微观尺寸的发光颗粒。用过氧化物酶标记大肠杆菌细胞，并通过添加基于鲁米诺的反应混合物诱导发光。电视图像中的发光斑点数量与标记细胞生长的细菌菌落数量高度一致。结果表明，我们的方法为临床诊断、食品微生物分析和环境评估等目的提供了一种快速简便的微生物计数系统。
