Miyamoto T, Kuramitsu Y, Ookuma A, Trevanich S, Honjoh K, Hatano S
Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
J Food Prot. 1998 Oct;61(10):1312-6. doi: 10.4315/0362-028x-61.10.1312.
A bioluminescence assay carried out with a photon-counting TV camera was evaluated for rapid enumeration of viable bacterial counts. The test sample was filtered through a membrane filter, and the membrane filter retaining bacteria was incubated at 37 degrees C for 6 h on a filter paper soaked with nutrient broth supplemented with 0.5% NaCl. The membrane filter was then subjected to a bioluminescence reaction, and the intensity of light and numbers of light emission points on the filter were measured with a photon-counting TV camera. The light intensity measured on seven different bacteria correlated with initial viable counts; the correlation coefficient was calculated to be 0.89. The number of light emission points measured on Escherichia coli also correlated with the initial viable counts (r = 0.81) in a range from 1 to 100 CFU. Presumptive bacterial counts by the present bioluminescence assay determined on 79 samples of vegetables and 122 samples of environmental water correlated well with the viable counts obtained by the conventional plating method, with correlation coefficients of 0.87 and 0.82, respectively.
利用光子计数电视摄像机进行的生物发光测定法被评估用于快速测定活菌数。将测试样品通过膜过滤器过滤，截留细菌的膜过滤器在补充有0.5%氯化钠的营养肉汤浸湿的滤纸上于37℃孵育6小时。然后使膜过滤器进行生物发光反应，并用光子计数电视摄像机测量过滤器上的光强度和发光点数量。在七种不同细菌上测得的光强度与初始活菌数相关；计算得出的相关系数为0.89。在1至100 CFU范围内，在大肠杆菌上测得的发光点数量也与初始活菌数相关（r = 0.81）。通过本生物发光测定法对79份蔬菜样品和122份环境水样进行的推测细菌计数与通过传统平板计数法获得的活菌数相关性良好，相关系数分别为0.87和0.82。
