Mahanty S K, Khaware R, Ansari S, Gupta P, Prasad R
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
FEMS Microbiol Lett. 1991 Nov 15;68(2):163-6. doi: 10.1016/0378-1097(91)90121-p.
Phosphate uptake studies in different strains of the dimorphic pathogenic yeast Candida albicans were undertaken to show that this yeast actively transported phosphate with an apparent Km in the range of 90-170 microM. The uptake was pH dependent and derepressible under phosphate starvation. Vanadate-resistant (van) mutants of C. albicans showed a 20-70% reduction in the rate of phosphate uptake in high phosphate medium and was associated with an increased Km and reduced Vmax. The magnitude of derepression under phosphate starvation was different between van mutants. These results demonstrate that van mutants may have developed resistance by modifying the rate of entry of vanadate.
对二态致病性酵母白色念珠菌的不同菌株进行了磷酸盐摄取研究，结果表明该酵母能主动转运磷酸盐，其表观米氏常数（Km）在90 - 170微摩尔范围内。摄取过程依赖于pH值，且在磷酸盐饥饿条件下可去阻遏。白色念珠菌的钒酸盐抗性（van）突变体在高磷酸盐培养基中磷酸盐摄取速率降低了20 - 70%，这与Km增加和Vmax降低有关。van突变体在磷酸盐饥饿条件下去阻遏的程度有所不同。这些结果表明，van突变体可能通过改变钒酸盐的进入速率而产生了抗性。
