Vogel R F, Lohmann M, Weller A N, Hugas M, Hammes W P
Institut für Lebensmitteltechnologie, Universität Hohenheim, Stuttgart, F.R.G.
FEMS Microbiol Lett. 1991 Nov 15;68(2):183-90. doi: 10.1016/0378-1097(91)90125-t.
Plasmid profiles of strains of Lactobacillus curvatus and L. sake isolated from meat or sauerkraut were analysed to investigate plasmid homology and distribution in relation to the ecology of these organisms in fermenting foods. A hybridisation probe was constructed by cloning of pLc2, a cryptic, 2.6-kbp plasmid from L. curvatus LTH683, into the Escherichia coli plasmid pRV50. In Southern hybridisations with the digoxygenine labeled pLc2 probe, pLc2-related small plasmids were frequently detected in meat-borne strains of L. casei subsp. pseudoplantarum, L. curvatus, L. sake, L. alimentarius, L. farciminis and L. halotolerans and in L. curvatus and L. sake isolated from sauerkraut. Among 27 Lactobacillus type strains originally isolated from habitats other than meat this type of homology was detected only with plasmids of L. buchneri and L. mali. Restriction-enzyme mapping of six small cryptic plasmids from L. curvatus and L. sake revealed strong structural homology but no similarity to previously characterized plasmids of lactobacilli. The presence of a variable region in addition to a conserved one and the occurrence of deletions during cloning of pLc2 suggest that vectors derived from these plasmids are likely to be structurally unstable.
分析从肉类或酸菜中分离出的弯曲乳杆菌和清酒乳杆菌菌株的质粒图谱，以研究质粒同源性及其与这些微生物在发酵食品中的生态学关系的分布情况。通过将来自弯曲乳杆菌LTH683的一个隐蔽的2.6kbp质粒pLc2克隆到大肠杆菌质粒pRV50中构建了一个杂交探针。在用地高辛标记的pLc2探针进行的Southern杂交中，在干酪乳杆菌假植物亚种、弯曲乳杆菌、清酒乳杆菌、食淀粉乳杆菌、发酵乳杆菌和耐盐乳杆菌的肉类携带菌株以及从酸菜中分离出的弯曲乳杆菌和清酒乳杆菌中经常检测到与pLc2相关的小质粒。在最初从肉类以外的生境中分离出的27株乳杆菌模式菌株中，仅在布氏乳杆菌和苹果乳杆菌的质粒中检测到这种同源性。对来自弯曲乳杆菌和清酒乳杆菌的六个小隐蔽质粒进行限制性酶切图谱分析，结果显示出很强的结构同源性，但与先前鉴定的乳杆菌质粒没有相似性。除了一个保守区域外还存在一个可变区域，以及在pLc2克隆过程中出现缺失，这表明源自这些质粒的载体在结构上可能不稳定。
