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比较使用rRNA探针和传统方法鉴定从肉类中分离出的清酒乳杆菌和弯曲乳杆菌菌株。

Comparison of the use of rRNA probes and conventional methods in identifying strains of Lactobacillus sake and L. curvatus isolated from meat.

作者信息

Nissen H, Dainty R

机构信息

MATFORSK Norwegian Food Research Institute, AS.

出版信息

Int J Food Microbiol. 1995 May;25(3):311-5. doi: 10.1016/0168-1605(94)00099-r.

DOI:10.1016/0168-1605(94)00099-r
PMID:7654516
Abstract

Published 23S rRNA directed probes for the species Lactobacillus sake and L. curvatus were used for rapid identification of lactic acid bacteria (LAB) isolated from meat. Of 96 strains of LAB from pork loin, 23 hybridised with the probe for L. sake and 16 with the probe for L. curvatus. Of 20 LAB strains from bologna, 19 strains hybridised with L. sake. By comparison with conventional biochemical tests, the probes provided a reliable identification.

摘要

已发表的用于清酒乳杆菌和弯曲乳杆菌物种的23S rRNA定向探针,用于从肉类中分离的乳酸菌(LAB)的快速鉴定。在从猪里脊肉分离的96株乳酸菌中,有23株与清酒乳杆菌探针杂交,16株与弯曲乳杆菌探针杂交。在从博洛尼亚香肠分离的20株乳酸菌中,有19株与清酒乳杆菌杂交。与传统生化测试相比,这些探针提供了可靠的鉴定。

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