Shandil R K, Kaur U, Vinayak V K
Department of Experimental Medicine, Postgraduate Institute of Medical Education & Research, Chandigarh.
Indian J Med Res. 1991 Sep;93:311-7.
A panel of 12 independent hybridoma cell lines secreting monoclonal antibodies to axenic E. histolytica (HM1) have been developed. A hybridoma cell line P4 C4 P2 F8 C8 (clone C8) produced monoclonal antibodies (MoAb C8) of IgG1 isotype which recognised a 29 KD surface associated antigen of amoebic trophozoites in Western immunoblot. Immunofluorescent probing with MoAb C8 employing live and acetone fixed amoebic trophozoites indicated 29 KD molecule on the surface plasma membrane of E. histolytica trophozoites. The MoAb C8 also agglutinated the live amoebic trophozoites. Pretreatment of amoebic trophozoites with anti 29 KD monoclonal antibody significantly (P less than 0.01) inhibited in vitro cytotoxicity of amoebic trophozoites to the cultured baby hamster kidney (BHK-21) cells. MoAb recognised a 29 KD molecule of E. histolytica trophozoites which mediated cytotoxic potentials of the parasite. The absence or variable degree of expression of cytotoxic 29 KD molecule may possibly serve as a marker to differentiate virulent/avirulent populations or strains of E. histolytica.
已经建立了一组12个独立的杂交瘤细胞系，这些细胞系分泌针对无菌溶组织内阿米巴（HM1）的单克隆抗体。杂交瘤细胞系P4 C4 P2 F8 C8（克隆C8）产生了IgG1同种型的单克隆抗体（单克隆抗体C8），该抗体在Western免疫印迹中识别出阿米巴滋养体的一种29 KD表面相关抗原。使用活的和经丙酮固定的阿米巴滋养体，用单克隆抗体C8进行免疫荧光检测表明，溶组织内阿米巴滋养体的表面质膜上存在29 KD分子。单克隆抗体C8也能凝集活的阿米巴滋养体。用抗29 KD单克隆抗体预处理阿米巴滋养体可显著（P小于0.01）抑制阿米巴滋养体对培养的幼仓鼠肾（BHK - 21）细胞的体外细胞毒性。单克隆抗体识别溶组织内阿米巴滋养体的一种29 KD分子，该分子介导了寄生虫的细胞毒性潜能。细胞毒性29 KD分子的缺失或表达程度的变化可能作为区分溶组织内阿米巴毒力/无毒力群体或菌株的一个标志物。
