Arisawa Tomiyasu, Tahara Tomomitsu, Shibata Tomoyuki, Nagasaka Mitsuo, Nakamura Masakatsu, Kamiya Yoshio, Fujita Hiroshi, Nakamura Masahiko, Yoshioka Daisuke, Arima Yuko, Okubo Masaaki, Hirata Ichiro, Nakano Hiroshi
Department of Gastroenterology, Fujita Health University School of Medicine, Toyoake 470-1192, Japan.
Int J Mol Med. 2007 Oct;20(4):539-44.
Macrophage migration inhibitory factor (MIF) is a key proinflammatory mediator, which plays a pivotal role in inflammatory and immune diseases. We attempted to clarify associations of the functional polymorphisms of the MIF gene promoter with the development of chronic gastritis. The study was performed with 290 stocked DNAs from subjects with no evidence of gastric malignancy. We employed the PCR-SSCP method to detect gene polymorphisms. The severity of histological chronic gastritis in antral biopsy specimens was classified according to the updated Sydney system. Both the 7/7-CATT repeat at position -794 and the -173 C/C genotypes were significantly associated with a risk of developing severe gastric mucosal atrophy (OR, 9.69; 95% CI, 1.29-72.5; and OR, 4.60; 95% CI, 1.05-20.2, respectively). In subjects younger than 60 years old, the number of 7-CATT alleles was significantly correlated with both the activity and inflammation scores (p=0.0079 and 0.0080, respectively). Our results suggested that functional promoter polymorphisms of the MIF gene might be associated with the severity of gastric mucosal inflammation in younger subjects and with the subsequent development of mucosal atrophy.
巨噬细胞移动抑制因子（MIF）是一种关键的促炎介质，在炎症和免疫疾病中起关键作用。我们试图阐明MIF基因启动子功能多态性与慢性胃炎发生之间的关联。本研究使用了290份来自无胃癌证据受试者的库存DNA。我们采用PCR-SSCP方法检测基因多态性。根据更新后的悉尼系统对胃窦活检标本中组织学慢性胃炎的严重程度进行分类。-794位的7/7-CATT重复序列和-173 C/C基因型均与发生严重胃黏膜萎缩的风险显著相关（OR分别为9.69；95%CI为1.29 - 72.5；以及OR为4.60；95%CI为1.05 - 20.2）。在60岁以下的受试者中，7-CATT等位基因数量与活动度评分和炎症评分均显著相关（p分别为0.0079和0.0080）。我们的结果表明，MIF基因启动子功能多态性可能与年轻受试者胃黏膜炎症的严重程度以及随后黏膜萎缩的发生有关。
