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体内叶绿素被皮秒光脉冲激发后的荧光寿命的强度依赖性。

Intensity Dependence of the Fluorescence Lifetime of in vivo Chlorophyll Excited by a Picosecond Light Pulse.

出版信息

Science. 1976 Jul 16;193(4249):227-9. doi: 10.1126/science.193.4249.227.

DOI:10.1126/science.193.4249.227
PMID:17796152
Abstract

New data on intensity-dependent lifetimes indicate that all previous in vivo fluorescence studies of chlorophyll by picosecond techniquies must be reinterpreted. Anomalously short lifetimes result from high-intensity effects due to exciton-excition annihilation processes. Measurements in Chlorella pyrenoidosa with single-pulse, low-intensity excitation indicate a longer "true" lifetime of 650 +/- 150 picoseconds.

摘要

新的强度相关寿命数据表明，以前所有利用皮秒技术进行的叶绿素体内荧光研究都必须重新解释。由于激子-激子湮灭过程的高强度效应，导致寿命异常缩短。用单脉冲、低强度激发对蛋白核小球藻进行的测量表明，其“真实”寿命较长，为 650 +/- 150 皮秒。

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Intensity Dependence of the Fluorescence Lifetime of in vivo Chlorophyll Excited by a Picosecond Light Pulse.体内叶绿素被皮秒光脉冲激发后的荧光寿命的强度依赖性。
Science. 1976 Jul 16;193(4249):227-9. doi: 10.1126/science.193.4249.227.
2
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引用本文的文献

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Light collection and harvesting processes in bacterial photosynthesis investigated on a picosecond time scale.在皮秒时间尺度上对细菌光合作用中的光收集和捕获过程进行了研究。
Proc Natl Acad Sci U S A. 1977 May;74(5):1997-2001. doi: 10.1073/pnas.74.5.1997.
2
Chlorophyll lasers: Stimulated light emission by chlorophylls and Mg-free chlorophyll derivatives.叶绿素激光器：叶绿素及无镁叶绿素衍生物的受激发光发射。
Proc Natl Acad Sci U S A. 1977 Jan;74(1):5-9. doi: 10.1073/pnas.74.1.5.
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Kinetics of excited states of pigment clusters in solubilized light-harvesting complex II: photon density-dependent fluorescence yield and transmittance.
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Biophys J. 1996 Dec;71(6):3370-80. doi: 10.1016/S0006-3495(96)79530-9.
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Lifetime of fluorescence from light-harvesting chlorophyll a/b proteins. Excitation intensity dependence.捕光叶绿素a/b蛋白的荧光寿命。激发强度依赖性。
Biophys J. 1981 Oct;36(1):193-201. doi: 10.1016/S0006-3495(81)84723-6.
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Bimolecular quenching of excitons and fluorescence in the photosynthetic unit.光合单元中激子与荧光的双分子猝灭
Biophys J. 1976 Dec;16(12):1447-52. doi: 10.1016/S0006-3495(76)85786-4.
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