捕光叶绿素a/b蛋白的荧光寿命。激发强度依赖性。
Lifetime of fluorescence from light-harvesting chlorophyll a/b proteins. Excitation intensity dependence.
作者信息
Nordlund T M, Knox W H
出版信息
Biophys J. 1981 Oct;36(1):193-201. doi: 10.1016/S0006-3495(81)84723-6.
Abstract
The fluorescence from a purified, aggregate form of the light-harvesting chlorophyll a/b protein has a lifetime of 1.2 +/- 0.5 ns at low excitation intensity, but the lifetime decreases significantly when the intensity of the 20-ps, 530-nm excitation pulse is increased above about 10(16) photons/cm2. A solubilized, monomeric form of the protein, on the other hand, has a fluorescence lifetime of 3.1 +/- 0.3 ns independent of excitation intensity from 10(14)-10(18) photons/cm2/pulse. We interpret the lifetime shortening in the aggregates and the lack of shortening in monomers in terms of exciton annihilation, facilitated in the aggregate by the larger population of interacting chlorophylls.
摘要
在低激发强度下，纯化的聚集体形式的捕光叶绿素a/b蛋白发出的荧光寿命为1.2±0.5纳秒，但当20皮秒、530纳米激发脉冲的强度增加到约10¹⁶光子/平方厘米以上时，寿命会显著缩短。另一方面，该蛋白的可溶单体形式的荧光寿命为3.1±0.3纳秒，与10¹⁴ - 10¹⁸光子/平方厘米/脉冲的激发强度无关。我们根据激子湮灭来解释聚集体中寿命的缩短以及单体中寿命未缩短的现象，在聚集体中，相互作用的叶绿素数量较多，这促进了激子湮灭。
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本文引用的文献
1
Intensity Dependence of the Fluorescence Lifetime of in vivo Chlorophyll Excited by a Picosecond Light Pulse.体内叶绿素被皮秒光脉冲激发后的荧光寿命的强度依赖性。
Science. 1976 Jul 16;193(4249):227-9. doi: 10.1126/science.193.4249.227.
2
The Light-harvesting Chlorophyll a/b-Protein Complex of Chlamydomonas reinhardii.莱茵衣藻的捕光叶绿素 a/b 蛋白复合物。
Plant Physiol. 1976 Jan;57(1):47-52. doi: 10.1104/pp.57.1.47.
3
Excitation lifetime of photosynthetic pigments in vitro and in vivo.光合色素在体外和体内的激发寿命。
Science. 1957 Mar 22;125(3247):555. doi: 10.1126/science.125.3247.555.
4
Picosecond fluorescence kinetics and fast energy transfer processes in photosynthetic membranes.光合膜中的皮秒荧光动力学和快速能量转移过程。
Biochim Biophys Acta. 1980 Dec 22;594(1):1-32. doi: 10.1016/0304-4173(80)90011-7.
5
Multiple excitations in photosynthetic systems.光合系统中的多重激发
Biophys J. 1976 Jan;16(1):87-91. doi: 10.1016/S0006-3495(76)85665-2.
6
Bimolecular quenching of excitons and fluorescence in the photosynthetic unit.光合单元中激子与荧光的双分子猝灭
Biophys J. 1976 Dec;16(12):1447-52. doi: 10.1016/S0006-3495(76)85786-4.
7
Picosecond laser study of fluorescence lifetimes in spinach chloroplast photosytem I and photosystem II preparations.菠菜叶绿体光系统I和光系统II制剂中荧光寿命的皮秒激光研究。
Biochim Biophys Acta. 1977 Mar 11;459(3):390-401. doi: 10.1016/0005-2728(77)90040-8.
8
The fluorescence decay kinetics of in vivo chlorophyll measured using low intensity excitation.使用低强度激发测量体内叶绿素的荧光衰减动力学。
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9
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10
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