Martin M A, Liebana J, Castillo A M, Gutierrez J, Piédrola G
Department of Microbiology, School of Medicine, University of Granada, Spain.
Microbios. 1991;67(272-273):195-202.
The sensitivity of 160 strains of Bacteroides fragilis, 74 beta-lactamase-positive and 86 negative, to two first generation and four second generation cephalosporins, alone and in combination with clavulanic acid and sulbactam, was investigated. For the susceptibility test the dilution method in agar was used. The detection of beta-lactamase production by this micro-organism was performed by means of the method using chromogenic cephalosporin of Nitrocefin. In both strains an important improvement in the activity of cephalothin, cephazolin, cefonicid and cefamandole was noted when they were combined with the two inhibitors. In combinations of cefoxitin and cefmetazole, no significant improvement was evident in the values of the parameters studied in the beta-lactamase-producing strains; and even in beta-lactamase-negative strains these values showed only a slight increase.
研究了160株脆弱拟杆菌（其中74株β-内酰胺酶阳性，86株阴性）对两种第一代和四种第二代头孢菌素单独使用以及与克拉维酸和舒巴坦联合使用时的敏感性。药敏试验采用琼脂稀释法。通过使用硝基头孢菌素的显色头孢菌素方法检测该微生物产生β-内酰胺酶的情况。在两种菌株中，当头孢噻吩、头孢唑林、头孢尼西和头孢孟多与两种抑制剂联合使用时，其活性有显著提高。在头孢西丁和头孢美唑的联合使用中，产β-内酰胺酶菌株所研究参数的值没有明显显著提高；即使在β-内酰胺酶阴性菌株中，这些值也仅略有增加。
