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[丁香假单胞菌(致坏死病变型和菜豆病变型)及禾本科假单胞菌(血清群VI)脂多糖的免疫化学特性]

[The immunochemical characteristics of the lipopolysaccharides of Pseudomonas syringae (pathovars atrofaciens and phaseolicola) and P. holci (serogroup VI)].

作者信息

Iakovleva L M, Zdorovenko G M, Gubanova N Ia, Gvozdiak R I

出版信息

Mikrobiol Zh (1978). 1991 May-Jun;53(3):9-14.

PMID:1779908
Abstract

Lipopolysaccharides (LPS) of the representatives of strains of serogroup VI Pseudomonas syringae (P. syringae pv. atrofaciens 2399, pv. phaseolicola 120a, 7842 and P. holci 8299) possessing virulence and confinement to the host-plant are characterized by high serological activity in direct and cross reactions of the binary diffusion in agar, immunoelectrophoresis, passive hemagglutination and inhibition of passive hemagglutination. A supernatant and a sediment obtained after ultracentrifugation of LPS preparations possessed O-antigenic activity. O-specific polysaccharide (PS) is serologically less active than the LPS preparations. Problems of the intergroup and intragroup serological affinity in connection with the structure of O-specific PS. It is proved that the basic chain of O-specific polysaccharide (D-rhamnane) plays definite (but not a single) part in displaying antigenic properties of the whole LPS macromolecule.

摘要

丁香假单胞菌血清群VI菌株(丁香假单胞菌致病变种2399、菜豆致病变种120a、7842以及霍氏假单胞菌8299)的脂多糖(LPS)具有致病性且局限于宿主植物,其特征在于在琼脂双向扩散、免疫电泳、被动血凝和被动血凝抑制的直接和交叉反应中具有高血清学活性。LPS制剂超速离心后获得的上清液和沉淀物具有O抗原活性。O特异性多糖(PS)的血清学活性低于LPS制剂。与O特异性PS结构相关的组间和组内血清学亲和力问题。已证明O特异性多糖的基本链(D-鼠李聚糖)在整个LPS大分子抗原特性的表现中起一定(但不是唯一)作用。

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