[细菌的抗原多糖。26. 来自樱桃假单胞菌467以及丁香假单胞菌丁香致病变种菌株218和P - 55的O - 特异性多糖的结构，这些菌株属于血清群II和III]

[Antigenic polysaccharides of bacteria. 26. Structure of O-specific polysaccharides from Pseudomonas cerasi 467 and Pseudomonas syringae pv. syringae strains 218 and P-55 belonging to serogroups II and III].

作者信息

Knirel' Iu A, Zdorovenko G M, Shashkov A S, Mamian S S, Iakovleva L M

出版信息

Bioorg Khim. 1988 Jan;14(1):82-91.

Abstract

Serologically active O-specific polysaccharides were obtained on mild acid hydrolysis of lipopolysaccharides from Pseudomonas cerasi 467 and Pseudomonas syringae pv. syringae strains 218 and P-55. On the basis of 1H- and 13C-NMR analysis, it was concluded that the P. cerasi polysaccharide has the following structure: ----3)-alpha-D-Rhap-(1----3)-alpha-D-Rhap-(1----2)-alpha-D-+ ++Rhap-(1---- which is identical to that of O-specific polysaccharide from P. syringae pv. morsprunorum C28 (Smith A. R. W. et al. Eur. J. Biochem., 1985, V. 149, No 1, p. 73-78). The polysaccharides from P. syringae pv. syringae strains possess the same backbone but differ by the presence of D-fucose as monosaccharide branches. Methylation and 1H- and 13C-NMR analysis revealed the following structure of these polysaccharides: (Formula: see text). The degree of substitution of the backbone trisaccharide units by the fucofuranose residues is about 35% for the strain 218 and about 85% for the strain P-55.

摘要

对来自樱桃假单胞菌467以及丁香假单胞菌丁香致病变种菌株218和P - 55的脂多糖进行温和酸水解后，获得了具有血清学活性的O - 特异性多糖。基于1H - 和13C - NMR分析，得出樱桃假单胞菌多糖具有以下结构：----3)-α - D - 鼠李糖基-(1----3)-α - D - 鼠李糖基-(1----2)-α - D - + ++鼠李糖基-(1---- ，这与来自李痘假单胞菌C28的O - 特异性多糖结构相同（史密斯A. R. W.等人，《欧洲生物化学杂志》，1985年，第149卷，第1期，第73 - 78页）。丁香假单胞菌丁香致病变种菌株的多糖具有相同的主链，但因存在作为单糖分支的D - 岩藻糖而有所不同。甲基化以及1H - 和13C - NMR分析揭示了这些多糖的以下结构：（分子式：见正文）。对于菌株218，呋喃岩藻糖残基对主链三糖单元的取代度约为35%，对于菌株P - 55约为85%。