L-谷氨酸脱羧酶的结构与功能
Structure and function of L-glutamate decarboxylase.
作者信息
Wu J Y, Huang W M, Reed-Fourquet L, Bao J, Nathan B, Wu E, Tsai W H
机构信息
Department of Physiology and Cell Biology, University of Kansas, Lawrence.
出版信息
Neurochem Res. 1991 Mar;16(3):227-33. doi: 10.1007/BF00966085.
Membrane bound L-glutamate decarboxylase (GAD) has been solubilized and partially purified from hog brain. The solubilized GAD appears to exist in two forms, alpha and beta, differing in their size and electrophoretic mobility. The alpha form has similar mobility as that of the soluble GAD in 7.5% and 5-25% gradient polyacrylamide gel electrophoresis suggesting that they are similar in size and charge. In addition, gene encoding for mouse brain GAD has been cloned and characterized. Mouse brain GAD cDNA consists of two DNA fragments with 1.6 and 1.0 Kb. The 1.6 and 1.0 Kb fragments contain 1657 and 974 bP, respectively. The significance of multiple forms of GAD is also discussed.
膜结合型L-谷氨酸脱羧酶（GAD）已从猪脑中溶解并部分纯化。溶解的GAD似乎以两种形式存在，α型和β型，它们的大小和电泳迁移率不同。在7.5%和5-25%梯度聚丙烯酰胺凝胶电泳中，α型的迁移率与可溶性GAD相似，这表明它们在大小和电荷方面相似。此外，已克隆并鉴定了编码小鼠脑GAD的基因。小鼠脑GAD cDNA由两个分别为1.6和1.0 Kb的DNA片段组成。1.6和1.0 Kb的片段分别包含1657和974个碱基对。还讨论了GAD多种形式的意义。
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