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一种改进的鸡胚内授精方法。

An improved procedure for intramagnal insemination of the chicken.

作者信息

Engel H N, Froman D P, Kirby J D

机构信息

Department of Animal Sciences, Oregon State University, Corvallis 97331.

出版信息

Poult Sci. 1991 Sep;70(9):1965-9. doi: 10.3382/ps.0701965.

Abstract

Intramagnal insemination is a useful technique in the analysis of spermatozoal function. Precise deposition of spermatozoa requires the use of laparotomy. However, hen-day egg production can be adversely affected by such a procedure. The present work demonstrates that postoperative hen-day egg production is affected by choice of anesthetic. Hens anesthetized with a mixture of ketamine and xylazine prior to laparotomy laid 14% fewer eggs (P less than .05) when compared with intact controls. In contrast, the postoperative hen-day egg production of hens anesthetized with xylazine alone was comparable with that of intact controls (P greater than .05). Furthermore, the use of xylazine alone increased the ease of handling sedated hens and decreased recovery time. Therefore, xylazine is recommended for anesthetizing hens prior to laparotomy.

摘要

体内受精是精子功能分析中的一项有用技术。精子的精确植入需要进行剖腹手术。然而,这种手术可能会对母鸡日产蛋量产生不利影响。目前的研究表明,剖腹手术后母鸡的日产蛋量受麻醉剂选择的影响。与完整对照组相比,在剖腹手术前用氯胺酮和赛拉嗪混合物麻醉的母鸡产蛋量减少了14%(P<0.05)。相比之下,仅用赛拉嗪麻醉的母鸡术后日产蛋量与完整对照组相当(P>0.05)。此外,仅使用赛拉嗪可提高对镇静母鸡的处理便利性并缩短恢复时间。因此,建议在剖腹手术前用赛拉嗪麻醉母鸡。

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