Zhang Jun-yi, Guo Qiao-sheng, Wei Xin-rong, Jiang Chuan-zhong
Institute of Chinese Medicinal Materials, Nanjing Agriculture University, Nanjing 210095, China.
Zhongguo Zhong Yao Za Zhi. 2007 Jun;32(12):1145-8.
To analyze the content of guanosine, total alkaloid and individual yield of Pinellia ternata from different populations in China and evaluate its quality.
Reversed-phase high-performance liquid chromatography (HPLC) was used to determine the content of guanosine. The content of alkaloid was determined by ultra violet spectrophometry. The results were analyzed by SPSS software.
The contents of guanosine and total alkaloid in P. ternata were 0.0136% -0.0264% and 0.0155% -0.0652% respectively. Individual yield was 0.5536-2.9740 g. All of the populations could be classified into 3 types through hierarchical cluster analysis.
There exist significant differences in the content of guanosine, total content of alkaloid and individual yield of P. ternata from different populations. It is suggested that breeding and selection for type II of P. ternata should be strengthened.
分析中国不同居群半夏中鸟苷含量、总生物碱含量及单株产量,评价其质量。
采用反相高效液相色谱法(HPLC)测定鸟苷含量。采用紫外分光光度法测定生物碱含量。结果用SPSS软件进行分析。
半夏中鸟苷和总生物碱含量分别为0.0136% - 0.0264%和0.0155% - 0.0652%。单株产量为0.5536 - 2.9740 g。通过系统聚类分析,所有居群可分为3种类型。
不同居群半夏的鸟苷含量、总生物碱含量及单株产量存在显著差异。建议加强半夏II型的选育。