Kessel D, Morgan A, Garbo G M
Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201.
Photochem Photobiol. 1991 Aug;54(2):193-6. doi: 10.1111/j.1751-1097.1991.tb02006.x.
Photosensitization by tin etiopurpurin (SnET2) was determined in cell culture using sensitizer dissolved in ethanol or solubilized via three different delivery systems: Cremophor EL, gamma-cyclodextrin or Molecusol (a more water-soluble cyclodextrin derivative). Sensitizer uptake was substantially more efficient when the delivery systems were employed, in terms of intracellular level needed to lethally photosensitize cells. We observed photodamage at both membrane and mitochondrial loci, but the former was better correlated with loss of viability.
使用溶解在乙醇中的敏化剂或通过三种不同的递送系统增溶的敏化剂（聚氧乙烯蓖麻油、γ-环糊精或莫雷西醇（一种水溶性更高的环糊精衍生物）），在细胞培养中测定了锡紫红素（SnET2）的光敏化作用。就使细胞发生致死性光敏化所需的细胞内水平而言，当采用递送系统时，敏化剂的摄取效率要高得多。我们在细胞膜和线粒体位点均观察到了光损伤，但前者与细胞活力丧失的相关性更好。
