Saha Nil Ratan, Bei Jin-Xin, Suetake Hiroaki, Araki Kyosuke, Kai Wataru, Kikuchi Kiyoshi, Lin Hao-Ran, Suzuki Yuzuru
Fisheries Laboratory, The University of Tokyo, Maisaka, Hamamatsu, Shizuoka 431-0214, Japan.
Fish Shellfish Immunol. 2007 Dec;23(6):1324-32. doi: 10.1016/j.fsi.2007.06.008. Epub 2007 Jun 29.
The primary structures of a CXC chemokine (CXCL8) and two CXC receptors (CXCR) have been characterized in fugu, Takifugu rubripes. Unlike mammalian and avian species, CXCL8 of teleosts including fugu lacks the ELR motif that appears to be important in ligand/receptor interactions on neutrophils. Genomic organization shows that fugu CXCL8 gene consists of four exons and three introns. As in other vertebrates, two CXCR genes isolated from fugu encode proteins CXCR1 and CXCR2 that possess characteristic seven transmembrane domains. Each receptor consists of two exons separated by an intron. Synteny analysis indicates that these two CXCRs were derived from whole genome duplication in teleosts, differing from mammalian CXCR1 and CXCR2. All of these genes are primarily expressed in the lymphoid tissues. Immune stimulation with PHA showed that the expression of both CXCL8 and CXCRs in PBL are upregulated even after only a short time period, but downregulated by LPS stimulation, implying that these genes are involved in the regulation of the immune response in fugu.
在红鳍东方鲀（Takifugu rubripes）中已鉴定出一种CXC趋化因子（CXCL8）和两种CXC受体（CXCR）的一级结构。与哺乳动物和鸟类不同，包括红鳍东方鲀在内的硬骨鱼类的CXCL8缺乏ELR基序，而该基序在中性粒细胞的配体/受体相互作用中似乎很重要。基因组结构显示，红鳍东方鲀CXCL8基因由四个外显子和三个内含子组成。与其他脊椎动物一样，从红鳍东方鲀中分离出的两个CXCR基因编码具有特征性七个跨膜结构域的蛋白质CXCR1和CXCR2。每个受体由两个被一个内含子隔开的外显子组成。共线性分析表明，这两个CXCR是硬骨鱼类全基因组复制的产物，与哺乳动物的CXCR1和CXCR2不同。所有这些基因主要在淋巴组织中表达。用PHA进行免疫刺激表明，即使在短时间后，PBL中CXCL8和CXCRs的表达也会上调，但在LPS刺激下会下调，这意味着这些基因参与了红鳍东方鲀免疫反应的调节。
