Kim Hyun-Jeong, Yasuike Motoshige, Kondo Hidehiro, Hirono Ikuo, Aoki Takashi
Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, 108-8477 Tokyo, Japan.
Fish Shellfish Immunol. 2007 Dec;23(6):1275-84. doi: 10.1016/j.fsi.2007.07.006. Epub 2007 Jul 20.
Chemokines are small, secreted cytokine peptides that have the ability to recruit a wide range of immune cells to sites of infection and disease. A novel CXC chemokine was obtained from Japanese flounder Paralichthys olivaceus. This chemokine cDNA contains an open reading frame of 333 nucleotides encoding 111 amino acid residues containing four conserved cysteine residues. The gene is composed of four exons and three introns as are those of mammalian and fish CXC chemokines. Results of homology and phylogenetic analysis revealed that the Japanese flounder CXC chemokine is closest to CXCL13 subgroup. The gene was expressed in immune-related organs, including head kidney, trunk kidney, spleen and peripheral blood leukocytes (PBLs). Japanese flounder CXC chemokine gene expression was observed at 3 and 6h after induction by LPS, but not at 3 and 6h after induction by poly I:C. These results suggest that the Japanese flounder CXC chemokine is probably associated with inflammatory as well as homeostatic functions.
趋化因子是一类小的、可分泌的细胞因子肽，能够将多种免疫细胞募集到感染和疾病部位。从日本牙鲆（Paralichthys olivaceus）中获得了一种新型CXC趋化因子。该趋化因子cDNA包含一个333个核苷酸的开放阅读框，编码111个氨基酸残基，其中含有四个保守的半胱氨酸残基。该基因由四个外显子和三个内含子组成，与哺乳动物和鱼类的CXC趋化因子相同。同源性和系统发育分析结果表明，日本牙鲆CXC趋化因子与CXCL13亚组最为接近。该基因在免疫相关器官中表达，包括头肾、躯干肾、脾脏和外周血白细胞（PBL）。在LPS诱导后3小时和6小时观察到日本牙鲆CXC趋化因子基因表达，但在poly I:C诱导后3小时和6小时未观察到。这些结果表明，日本牙鲆CXC趋化因子可能与炎症以及稳态功能有关。
