Johnson C B, Holman R T
The Hormel Institute, University of Minnesota, Austin, Minnesota.
Lipids. 1966 Nov;1(6):371-80. doi: 10.1007/BF02532539.
The mass spectra of 1- and 2-monoglycerides, their diacetyl derivatives and their trimethylsilyl (TMS) ether derivatives were recorded at high (80 eV) and low (6-13 eV) voltages. The fatty acid components of these derivatives included the even-numbered saturated acids from capric to arachidic acid plus oleic, linoleic and linolenic acids. Differences between isomeric 1- and 2-monoglycerides were not sufficient to provide a basis for the analysis of these isomers. Mass spectra of the monoglycerides were very similar to the corresponding methyl esters. Mass spectra of the diacetyl derivatives were qualitatively similar to triglycerides of long-chain fatty acids, but parent ions were not observed. The spectra of diacetyl derivatives may be used for distinguishing 1- and 2-monoglycerides, but the spectra of the TMS ethers are better in this regard. The latter derivatives have fragmentation patterns distinct for the 1- and 2-monoglyceride isomers, particularly at low electron voltages.
在高电压（80电子伏特）和低电压（6 - 13电子伏特）下记录了1 - 单甘油酯、2 - 单甘油酯、它们的二乙酰衍生物以及它们的三甲基硅烷基（TMS）醚衍生物的质谱。这些衍生物的脂肪酸成分包括从癸酸到花生酸的偶数饱和酸，以及油酸、亚油酸和亚麻酸。异构体1 - 单甘油酯和2 - 单甘油酯之间的差异不足以作为分析这些异构体的基础。单甘油酯的质谱与相应的甲酯非常相似。二乙酰衍生物的质谱在定性上与长链脂肪酸甘油三酯相似，但未观察到母离子。二乙酰衍生物的质谱可用于区分1 - 单甘油酯和2 - 单甘油酯，但在这方面TMS醚的质谱更好。后一种衍生物对于1 - 和2 - 单甘油酯异构体具有独特的裂解模式，特别是在低电子电压下。
