Greif R L, Eich E F
Metabolism. 1977 Aug;26(8):851-6. doi: 10.1016/0026-0495(77)90003-8.
Thyroids of goitrogen-treated rats contain increased amounts of a protein inhibitor of ribonuclease activity at pH greater than 7 (alkaline ribonuclease inhibitor, ARI). We report here that thyroids from hyperthyroid patients contain more ARI than normal human thyroids. This increase parallels RNA concentration. The inhibitor is heat labile, inactivated by sulfhydryl blocking agents, and has a molecular weight near 50, 000 daltons. ARI is quantitated by its activity against bovine pancreatic RNase, but it also inhibits human thyroid RNase. Analyses of a solitary toxic nodule and its surrounding suppressed tissue confirm in tissues from a single patient our results in tissue from numbers of thyrotoxic and euthyroid individuals and decrease the likelihood that changes are induced by antithyroid medication. A possible regulatory role for ARI is suggested.
用致甲状腺肿物质处理的大鼠甲状腺中，在pH大于7时，核糖核酸酶活性的蛋白质抑制剂（碱性核糖核酸酶抑制剂，ARI）含量增加。我们在此报告，甲状腺功能亢进患者的甲状腺比正常人的甲状腺含有更多的ARI。这种增加与RNA浓度平行。该抑制剂对热不稳定，可被巯基阻断剂灭活，分子量接近50000道尔顿。ARI通过其对牛胰腺核糖核酸酶的活性进行定量，但它也抑制人甲状腺核糖核酸酶。对单个毒性结节及其周围受抑制组织的分析，在来自单个患者的组织中证实了我们在许多甲状腺毒症和甲状腺功能正常个体的组织中的结果，并降低了变化是由抗甲状腺药物诱导的可能性。提示ARI可能具有调节作用。
