Science. 1986 Jun 6;232(4755):1253-5. doi: 10.1126/science.232.4755.1253.
Uncultivable for more than 25 years, the sex-ratio spiroplasma of Drosophila willistoni grew in a tissue culture medium (H-2) containing an embryo-derived lepidopteran cell line (IPLB-TN-R(2)). After adaptation, it grew in a cell-free H-2 medium. This success demonstrates the usefulness of cell culture systems for cultivation of fastidious microorganisms and facilitates study of the sex-ratio trait in Drosophila.
25 年以上无法培养的威斯塔果蝇性比螺旋体在含有来源于胚胎的鳞翅目细胞系（IPLB-TN-R(2)）的组织培养基（H-2）中生长。适应后，它在无细胞的 H-2 培养基中生长。这一成功证明了细胞培养系统对于培养难养微生物的有用性，并促进了对果蝇性比性状的研究。
