一种针对苛求性螺旋体的特定培养基。

A defined medium for a fastidious Spiroplasma.

作者信息

Hackett K J, Ginsberg A S, Rottem S, Henegar R B, Whitcomb R F

出版信息

Science. 1987 Jul 31;237(4814):525-7. doi: 10.1126/science.3603039.

Abstract

A defined medium (H-1) was developed for cultivation of the suckling mouse cataract agent, Spiroplasma mirum, a fastidious member of the class Mollicutes that causes cataracts and chronic brain infection in inoculated neonate mice. The H-1 medium was used to show the importance of sphingomyelin as a growth factor for the culture of the spiroplasma in vitro. The growth of Spiroplasma mirum and the pathology it induces in sphingomyelin-rich tissues in vivo may be related to this dependency.

摘要

已开发出一种特定培养基（H-1），用于培养乳鼠白内障病原体——微小螺旋体，它是支原体纲中的一种苛求菌，可在接种的新生小鼠中引起白内障和慢性脑部感染。H-1培养基用于证明鞘磷脂作为体外培养该螺旋体的生长因子的重要性。微小螺旋体的生长及其在体内富含鞘磷脂的组织中诱发的病理学变化可能与此依赖性有关。