Ueda H, Doi Y, Yoshizuka M, Sakamoto Y, Mori N, Araki H, Fujimoto S
Department of Anatomy, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan.
Acta Anat (Basel). 1991;142(1):62-9. doi: 10.1159/000147162.
Dose-dependent contractions of the in vitro swine coronary artery were induced by application of histamine and acetylcholine, but not of angiotensin II, ergonovine, noradrenaline, prostaglandin F2 alpha and serotonin. Ultrastructural changes especially of the tunica intima during the contractions were observed at 2, 5 and 30 min after application of histamine and acetylcholine. The intimal gutter spirally running along the longitudinal axis of the vessel was obscured, and the intimal surface became extensively indented. Exclusively in the histamine-treated samples, the increase in number and size of the intracellular vacuoles and the dilation of the intercellular clefts to the extent of the intercellular vacuoles were observed in the endothelium. Moreover, the enhancement of the endothelial permeability was indicated by the marker experiments using horseradish peroxidase. Such endothelial cell damages and the enhancement of the endothelial permeability may amplify the coronary artery contraction.
组胺和乙酰胆碱可诱导体外猪冠状动脉产生剂量依赖性收缩，但血管紧张素II、麦角新碱、去甲肾上腺素、前列腺素F2α和5-羟色胺则不能。在应用组胺和乙酰胆碱后2分钟、5分钟和30分钟时，观察到收缩过程中尤其是内膜的超微结构变化。沿血管纵轴呈螺旋状走行的内膜沟模糊不清，内膜表面出现广泛凹陷。仅在组胺处理的样本中，在内皮细胞中观察到细胞内空泡数量和大小增加以及细胞间隙扩张至细胞内空泡的程度。此外，使用辣根过氧化物酶的标记实验表明内皮通透性增强。这种内皮细胞损伤和内皮通透性增强可能会放大冠状动脉收缩。
