Kloss P, Bernstein H G, Kirschke H, Wiederanders B
Institute of Anatomy, Medical Academy, Magdeburg, FRG.
Acta Anat (Basel). 1991;142(2):138-40. doi: 10.1159/000147178.
Cathepsin B was localized in multiple neurons of the rat central nervous system by means of the peroxidase-antiperoxidase technique and immunogold labeling using a polyclonal antiserum produced in rabbits against rat liver enzyme. The main intracellular locus of cathepsin B antigenic sites was in lysosomes. In some cases, however, immunoreactive material was also detected outside lysosomes (i.e. at the membranes of the rough endoplasmic reticulum). The findings are discussed with respect to the proposed role of the enzyme in the general protein metabolism of the brain and the potency of the antiserum to label the proform of cathepsin B.
通过过氧化物酶-抗过氧化物酶技术和免疫金标记法，使用兔抗大鼠肝脏酶产生的多克隆抗血清，组织蛋白酶B定位在大鼠中枢神经系统的多个神经元中。组织蛋白酶B抗原位点的主要细胞内定位是在溶酶体中。然而，在某些情况下，在溶酶体之外（即糙面内质网的膜上）也检测到了免疫反应性物质。结合该酶在大脑一般蛋白质代谢中的假定作用以及抗血清标记组织蛋白酶B前体形式的效力对这些发现进行了讨论。
