Bernstein H G, Sormunen R, Järvinen M, Kloss P, Kirschke H, Rinne A
Institute of Anatomy, Medical Academy, Magdeburg, GDR.
J Hirnforsch. 1989;30(3):313-7.
The regional distribution and cellular localization of the lysosomal proteinase cathepsin B was studied by use of monospecific antiserum. The application of the peroxidase-antiperoxidase technique at the light microscopic level revealed cathepsin B immunoreactive neurons in many brain areas. A strong immunoreaction was found in pyramidal cells of the cortex, large neurocytes of the septal region, some hippocampal neurons and magnocellular nerve cells of the hypothalamus. Immunogold labeling on ultrathin cryosections of rat neocortex revealed the enzyme protein to be associated with lysosomes.
利用单特异性抗血清研究了溶酶体蛋白酶组织蛋白酶B的区域分布和细胞定位。在光学显微镜水平应用过氧化物酶-抗过氧化物酶技术，发现在许多脑区存在组织蛋白酶B免疫反应性神经元。在皮质锥体细胞、隔区大神经细胞、一些海马神经元和下丘脑大细胞神经细胞中发现了强烈的免疫反应。大鼠新皮质超薄冰冻切片上的免疫金标记显示该酶蛋白与溶酶体相关。
