Science. 1983 Feb 11;219(4585):722-7. doi: 10.1126/science.219.4585.722.
Performance of enzymes and whole cells in commercial applications can often be dramatically improved by immobilization of the biocatalysts, for instance, by their covalent attachment to or adsorption on solid supports, entrapment in polymeric gels, encapsulation, and cross-linking. The effect of immobilization on enzymatic properties and stability of biocatalysts is considered. Applications of immobilized enzymes and cells in the chemical, pharmaceutical, and food industries, in clinical and chemical analyses, and in medicine, as well as probable future trends in enzyme technology are discussed.
通过将生物催化剂（例如，通过共价附着或吸附在固体载体上、包埋在聚合物凝胶中、封装和交联）固定化，可以显著提高酶和整个细胞在商业应用中的性能。讨论了固定化对生物催化剂酶性质和稳定性的影响。讨论了固定化酶和细胞在化学、制药和食品工业、临床和化学分析以及医学中的应用，以及酶技术的未来可能趋势。
