Science. 1984 Oct 5;226(4670):53-5. doi: 10.1126/science.226.4670.53.
An assay has been developed to measure the rate of transposition of the transposable element Ty in Saccharomyces cerevisiae. The assay is based on the altered expression of the glucose-repressible alcohol dehydrogenase gene of yeast upon insertion of a Ty in front of this gene. By this assay the transposition rate of Ty elements was found to increase approximately 100-fold at temperatures lower than 30 degrees C, the optimum growth temperature for Saccharomyces cerevisiae.
现已开发出一种测定酵母 Ty 可移动元件转座速率的方法。该测定法基于 Ty 插入酵母葡萄糖抑制型醇脱氢酶基因的前端，导致该基因表达改变。用此方法发现 Ty 元件的转座速率在低于最适酵母生长温度 30°C 的温度下增加约 100 倍。
