Science. 1985 Oct 25;230(4724):450-3. doi: 10.1126/science.230.4724.450.
In cells of Chlamydomonas reinhardtii y-1 kept in the dark, 1,7-phenanthroline stimulated the conversion of protochlorophyllide to chlorophyllide b. A membrane fraction was obtained from degreened cells that was active in this conversion only when phenanthroline was present. Untreated cells excreted protochlorophyllide, which was used as substrate for this in vitro reaction. This system may provide a clue to how chlorophyllide b is synthesized in plant cells.
在黑暗中培养的莱茵衣藻 y-1 细胞中，1,7-菲咯啉能刺激原叶绿素酸酯向叶绿素 b 的转化。从退绿细胞中得到一个膜部分，只有当存在菲咯啉时，该膜部分在此转化中才具有活性。未经处理的细胞会分泌原叶绿素酸酯，该物质可用作体外反应的底物。该系统可能为阐明叶绿素 b 在植物细胞中如何合成提供了线索。
